What is EDC crosslinking?

What is EDC crosslinking?

1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC or EDAC) is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

What is EDC NHS?

EDC (carbodiimide) crosslinking reaction scheme. Carboxyl-to-amine crosslinking with the popular carbodiimide, EDC. N-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS) is often included in EDC coupling protocols to improve efficiency or create dry-stable (amine-reactive) intermediates.

Is EDC water sensitive?

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble carbodiimide usually handled as the hydrochloride.

Can phosphate groups be crosslinked?

In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The crosslinking efficiency achieved 30%. The approaches developed can be employed in the studies of pro- and eucaryotic homologs of the E. coli Fpg protein as well as other repair enzymes.

How do I prepare for EDC NHS?

Protocol

1. Equilibrate the EDC and NHS to room temperature before opening.
2. Prepare 1ml of a 1mg/ml solution of Protein #1 in Conjugation Buffer 1.
3. Add 0.4mg EDC and 0.6mg NHS or 1.1.mg sulfo-NHS and react for 15 minutes at room temperature.
4. Add 1.2µl 2-mercaptoethanol to quench the EDC.

Is NHS soluble in water?

Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents. Activation with NHS, however, decreases water- solubility of the modified carboxylate molecule, while activation with Sulfo-NHS preserves or increases water-solubility of the modified molecule, by virtue of the charged sulfonate group.

Is REDCap an EDC?

REDCap Cloud is a 4th generation EDC solution with transformational built in features including Mobile App Based EDC, Mid-Study Changes, Monitoring, Query Management, Medical Coding, Custom Randomization, and a File Repository.

What is CRF tracking?

Abstract: A case report form (CRF) tracking system helps ensure all required CRFs are collected. The system may also be useful in determining which monitors or sites are having trouble completing or collecting the CRFs in a timely manner.