What is the sequence for HindIII?

What is the sequence for HindIII?

Under the standard reaction conditions, the restriction endonuclease HindIII cleaves double-stranded DNA, within the recognition sequence–A/AGCTT–at the position indicated by the arrow.

What is the DNA sequence HindIII uses?

The restriction enzyme HindIII cuts DNA at the sequence AAGCTT, and the restriction enzyme HpaII cuts DNA at the sequence CCGG. For HpaII, the frequency of the four-base-long sequence is (1/4)4, or 1/256. The average spacing between HpaII sites is approximately 0.25 kb.

Where did HindIII get name?

Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism.

How does HindIII get its name?

What is λ HindIII used for?

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.

Which is the best multiple sequence alignment program?

Multiple Sequence Alignment. Clustal Omega is a new multiple sequence alignment program that uses seeded guide trees and HMM profile-profile techniques to generate alignments between three or more sequences.

How does the Hin DIII restriction process work?

Hin dIII restrictions process results in formation of overhanging palindromic sticky ends. The cleavage of this sequence between the AA’s results in 5′ overhangs on the DNA called sticky ends : Restriction endonucleases are used as defense mechanisms in prokaryotic organisms in the restriction modification system.

How big of a file do I need to align two sequences?

For the alignment of two sequences please instead use our pairwise sequence alignment tools. Important note: This tool can align up to 4000 sequences or a maximum file size of 4 MB. The default settings will fulfill the needs of most users. More options…

How to align two or more sequences in blast?

Align two or more sequences [?] Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.