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What is the pKa of bromophenol blue?

What is the pKa of bromophenol blue?

3.0 to 4.6
Acid-Base Indicators

Indicator pH Range pKa
Methyl yellow 2.9 to 4.0 3.3
Methyl orange 3.1 to 4.4 3.4
Bromophenol blue 3.0 to 4.6 3.85
Bromocresol green 4.0 to 5.6 4.7

What is bromophenol blue indicator used for?

Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis it can be used as a color marker and as as a biological stain it can be used to stain proteins and nucleic acids.

What is bromophenol blue soluble in?

Bromophenol blue is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of bromophenol blue in ethanol is approximately 10 mg/ml and approximately 30 mg/ml in DMSO and DMF.

What is the color of bromophenol blue?

Bromophenol Blue is a pH indicator dye that turns yellow under acidic conditions. Bromophenol Blue has a pI below pH 4.0. The concentrated dye solution may have a reddish or greenish cast to the color.

What is the role of Bromo phenol blue in electrophoresis?

It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

What is the pKa of phenol why is its pKa value lower than that of an alcohol?

While in ethanol, ethyl group has a + I effect and increases the electron density around H of O—H group making it difficult to remove H as H+. Hence, phenol is more acidic than ethanol and has lower pKa value than ethanol.

A pKa of 4.0(1) indicates bromophenol blue will exist almost entirely in the anion form at pH values of 5 to 9 and therefore volatilization from water surfaces is not expected to be an important fate process(2).

What is the Koc of bromophenol blue?

Using a structure estimation method based on molecular connectivity indices(1), the Koc of bromophenol blue can be estimated to be 1.5X10+6(SRC). According to a classification scheme(2), this estimated Koc value suggests that bromophenol blue is expected to be immobile in soil. The pKa of bromophenol blue is 4.0(3),…

What is the molecular formula for bromophenol blue?

Bromophenol blue PubChem CID 8272 Structure Find Similar Structures Molecular Formula C19H10Br4O5S Synonyms Bromophenol blue 115-39-9 BROMPHENOL BLU Molecular Weight 670.0

Why does bromophenol blue not undergo hydrolysis?

Bromophenol blue is not expected to undergo hydrolysis in the environment due to the lack of functional groups that hydrolyze under environmental conditions(1). Bromophenol blue has a maximum absorbance wavelength of 598 nm(2) and therefore may be susceptible to direct photolysis by sunlight(SRC).

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