What does the Vacufuge do?

What does the Vacufuge do?

This protocol describes the use of the Eppendorf vacufuge to assist in drying Biomatrica plates for long term storage of DNA extracts. DNA extracts will be transferred from original extract tubes or plates either manually or utilizing a liquid handling robot onto Biomatrica plates for long-term storage.

What is vacuum concentrator?

Used in proteomics, genomics, cell biology, microbiology, and drug development, a vacuum centrifuge concentrator uses a vacuum, centrifugal force, and sometimes heat or gas blow down to evaporate liquid and concentrate DNA, RNA, nucleotides, and other proteins for further use or study. …

How does a vacuum centrifuge work?

In a vacuum concentrator, samples are held in a rotor that spins at 1700 RPM creating a centrifugal force. The centrifugal force prevents liquids from bumping out of the tube when vacuum is applied. Heat is applied indirectly through the walls of the vacuum chamber.

How does a lyophilizer work?

A lyophilizer executes a water removal process typically used to preserve perishable materials, to extend shelf life or make the material more convenient for transport. Lyophilizers work by freezing the material, then reducing the pressure and adding heat to allow the frozen water in the material to sublimate.

How long does Speed Vac take?

I always heat up the speed vac to 60 degree C. Drying the peptide in 400 ul of 50% ACN takes about an hour.

What is Speed Vac concentrator?

Thermo Scientific Savant SpeedVac vacuum concentrators, refrigerated vapor traps and pumps use state-of-the-art centrifugation, vacuum and heat technologies for removing solvents and concentrating samples while maintaining sample integrity.

How does a centrifugal concentrator work?

Centrifugal concentrators work by removing solvent under vacuum, so they’re effectively boiling the solvent at a very low temperature. As the pressure in a system decreases, the boiling point of a solvent also decreases, allowing the solvent to boil and turn to a gas at a very low temperature.

How is centrifugation done?

Centrifugation is a technique used for the separation of particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. The particles are suspended in a liquid medium and placed in a centrifuge tube. The tube is then placed in a rotor and spun at a define speed.

How does freeze dry machine work?

Raw or cooked foods are placed in the freeze dryer where they are frozen to between -30 degrees and -50 degrees Fahrenheit. Once frozen, the freeze dryer creates a vacuum in the food chamber. 3. As the food is gradually warmed, the water turns to vapor and evaporates out of the food (sublimation).

How does freeze dried work?

Freeze-drying is a special form of drying that removes all moisture and tends to have less of an effect on a food’s taste than normal dehydration does. In freeze-drying, food is frozen and placed in a strong vacuum. The water in the food then sublimates — that is, it turns straight from ice into vapor.

How do I turn off SpeedVac?

Older SpeedVac Systems – These all have their mains switches located on the right side of the units. Both the SC210 and the SC250EXP do not have a main’s switch and can only be powered off by un-plugging the system from its power source. The RVT5105 has a mains power switch located on the left side panel.

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