What are RAW macrophages?

What are RAW macrophages?

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products.

What is raw264 cell line?

ECACC General Cell Collection: RAW 264 Cell Line Description: Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abelson Leukaemia virus (A-MuLV). Cells with pinocytose neutral red and phagocytose zymosan.

How do RAW cells passage?

Passage Procedure

1. Transfer RAW 264.7 growth medium 1 (RAWGM1) to a T150 flask (100 ml of growth medium per flask).
2. Place flask in incubator at 37 °C with a 5% CO2 atmosphere for at least 30 min to equilibrate the medium.
3. Enter the bar code of the cells into the cell line GUI.

Can you culture macrophages?

For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, the macrophages can be harvested and seeded into culture wells at required cell densities for carrying out experiments.

Are RAW cells macrophages?

The RAW 264.7 cells are monocyte/macrophage-like cells, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice. These cells are being described as an appropriate model of macrophages. They are capable of performing pinocytosis and phagocytosis.

What are RAW264 7 macrophages?

In addition to primary cells, the RAW264. 7 cells are monocyte/macrophage like cell linage, originating from Abelson leukemia virus transformed cell linage derived from BALB/c mice. 7 cells have been described as an appropriate model of macrophages, as the cell are capable for performing pinocytosis and phagocytosis.

What are RAW264 7?

Description: The RAW264. 7 cell line was established from a tumor induced by the Abelson murine leukemia virus. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets.

Are RAW cells adherent or suspension?

Subculture Routine Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask.

How do you make monocytes derived macrophages?

Tissue macrophages can be derived from monocytes. When isolated from blood and cultured in media with serum, adherent monocytes will differentiate into macrophages. For a pure macrophage culture, we recommend that you add factors such as M-CSF.

Why are RAW 264.7 cells used?

The RAW 264.7 cells are monocyte/macrophage-like cells, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice. These cells are being described as an appropriate model of macrophages. Upon LPS stimulation RAW 264.7 cells increase nitric oxide (NO) production and enhance phagocytosis.